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Jackson Immuno org d ow
Org D Ow, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/org+d+ow/Biotin-SP+(long+spacer)+AffiniPure+F(ab')%E2%82%82+Fragment+Donkey+Anti-Human+IgM%2C+Fc5%CE%BC+fragment+specific/10__1128_slash_jcm__01836___20-125-62-68
Average 93 stars, based on 8 article reviews
org d ow - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Incubation:

Article Title: Serum Epitope Repertoire Analysis Enables Early Detection of Lyme Disease with Improved Sensitivity in an Expandable Multiplex Format
Article Snippet: .. For IgM isotype screening the protocol was adjusted as 297 follows: 1) after serum incubation with the library, E. coli cells were centrifuged, the 298 supernatant removed, and the cells were resuspended in 500 uL 1x PBS containing a 1:100 299 dilution of biotin-SP (long-spacer) conjugated donkey anti-Human IgM secondary antibody 300 on N ovem ber 30, 2020 by guest http://jcm .asm .org/ D ow nloaded from 15 (Jackson Immunoresearch, 709-066-073). .. 2) The plate was incubated for 1 hour at 4 C with 301 orbital shaking (800 rpm), the cells were again centrifuged, supernatant removed, and cells 302 were resuspended in 900 uL of 1x PBS + 100 uL of Dynabeads MyOne streptavidin T1 coated 303 magnetic beads (ThermoFisher Scientific, 65601).

Staining:

Article Title: Adeno-associated Virus (AAV) Assembly-Activating Protein Is Not an Essential Requirement for Capsid Assembly of AAV Serotypes 4, 5, and 11
Article Snippet: .. For AAP localization, the cells were 242 stained with mouse monoclonal anti-FLAG M2 antibody (F1804; Sigma-Aldrich, St. 243 Louis, MO) and rabbit polyclonal anti-nucleostemin antibody (sc-67012; Santa Cruz 244 Biotechnology, Dallas, TX), followed by DAPI (4’,6-diamidino-2- phenylindole), Alexa 245 Fluor 488-AffiniPure goat anti-mouse IgG antibody (115-545-166; Jackson 246 ImmunoResearch, West Grove, PA), and Cy3-AffiniPure goat anti-rabbit IgG antibody 247 on N ovem ber 18, 2016 by U N IV O F O T A G O http://jvi.asm .org/ D ow nloaded from 12 (111-165-144; Jackson ImmunoResearch). .. For imaging of AAV capsids, cells were 248 stained with mouse monoclonal anti-AAV2 capsid antibody (A20 clone, 03-61055; 249 American Research Products Inc., Waltham, MA), mouse monoclonal anti-AAV4 capsid 250 antibody (ADK4 clone, 03-610147; American Research Products Inc.), mouse 251 monoclonal anti-AAV5 capsid antibody (ADK5a clone, 03-61048; American Research 252 Products Inc.), mouse monoclonal anti-AAV8 capsid antibody (ADK8 clone, 03-651160; 253 American Research Products Inc.), or mouse monoclonal anti-AAV8/9 capsid antibody 254 (ADK8/9 clone, 03-651161; American Research Products Inc.) and rat monoclonal anti-255 DYKDDDDK (FLAG) antibody (NBP1-06712; Novus Biological, Littleton, CO), 256 followed by DAPI, Alexa Fluor 488-AffiniPure goat anti-mouse IgG antibody (115-545-257 166; Jackson ImmunoResearch) and Cy3-AffiniPure donkey anti-rat IgG antibody (712-258 165-153; Jackson ImmunoResearch).



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